Human Kidney Proximal Tubule-on-a-chip for Drug Trans- Porter Studies and Nephrotoxicity Assessment
نویسندگان
چکیده
We introduce a simple microfluidic device that mimics function of the kidney proximal tubule by integrating a polydimethylsiloxane (PDMS) microfluidic channel and human proximal tubule cells on a porous extracellular matrix (ECM)coated porous membrane. This allows us to culture the kidney epithelial cells under a low level (0.2 dyne/cm) of fluid shear stress that mimics that of the normal kidney tubule. By providing in this system, a more physiological microenvironment, we are able to enhance epithelial cell polarization, cytoskeletal reorganization, primary cilia formation and response to renal toxins. These results suggest that our system is useful for studying human-relevant renal toxicity, and has the potential for drug development and advanced tissue engineering applications.
منابع مشابه
Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment.
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